Bactericidal potential of plant extracts and green synthesized silver nanoparticles against antibiotic resistant soil borne bacterial pathogens

Abstract

The purpose of this study was to identify and filter harmful microorganisms from Shahdara Lahore's soil. To determine whether the isolated strains were pathogenic, the blood agar test was performed. The pathogenicity of each strain was established by the presence of beta hemolysis. It has been determined how sensitive harmful bacteria are to different plant extracts and nanoparticles. All of the nanoparticles exhibited good antibacterial activity, as indicated by their inhibition zone, and there was no significant variation in their antibacterial activity (p>0.05). Plant extracts from Azadirachta indica and Eucalyptus radiata have demonstrated strong antibacterial action against isolated pathogens. It has been determined that pathogenic bacteria are resistant to a variety of antibiotics. The one-way Anova results indicated a significant difference (p <0.05) in the values of the various antibiotics. While the antibacterial activity of all the other antibiotics was identical, Tukey and Bonferroni test through post-hoc analysis revealed that the value of chloramphenicol was significantly different. The antibacterial activity of all antibiotics was good, with the exception of chloramphenicol, which was less effective. The investigation came to the conclusion that Shahdara Lahore's soil has a variety of harmful bacteria that can infect both humans and animals. Plants are also harmed by these soil-dwelling pathogenic microorganisms. Antibiotic resistance in bacteria has been identified as a result of overuse and misuse of these medications to treat infections. To prevent pathogens from becoming resistant, it is advised that different biological tools, such as plant extracts and nanoparticles, be investigated more frequently as antibacterial methods.