Assessment Of Self Emulsifying Drug Delivery System Loaded With Curcumin Against Enterococcus Faecalis In In Vitro Denture Model
DOI:
https://doi.org/10.63075/eks53480Keywords:
E. Faecalis, SEDDS, Acrylic Dentures, Curcumin, Biofilm, MIC, MBC and in Vitro Biofilm.Abstract
Resistance due to misuse of antibiotics needs to tackled with alternative herbal treatments. Enterococcus faecalis infections is a leading cause of dental caries because of its virulence factors promoting it form biofilm and multidrug resistance. Curcumin being an antibacterial herb was further enhanced by self-emulsifying drug delivery systems (SEDDS) against. This study evaluates the antibacterial and antibiofilm activity of curcumin-loaded SEDDS against E. faecalis isolates in in-vitro model of dental caries. Two formulations (F1 and F2) were prepared with various excipients. Physicochemical compatibility was assessed using Zeta sizer. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and antibiofilm activity were determined. F1 exhibited a PDI of 0.148, zeta potential of -6.70 mV, and a size of 30.52 nm, while F2 showed values of 0.159, -5.62 mV, and 72.0 nm respectively. MIC for F1 was 300µg/mL and MBC was 150µg/mL. Antibiofilm assays revealed F1 and F2 inhibited biofilm formation by E. faecalis isolates, with F1 inhibiting 66⁒ to 71.2⁒ and F2 inhibiting 66.9⁒ to 72.6⁒ at the highest curcumin concentration.
