Characterization And Antifugal Susceptibility Of Different Fungal Strains Isolated From Hospital Environment

Abstract

Nosocomial fungal infections pose a growing global public health risk, necessitating immediate attention and effective management. Due to the limited availability of treatment options, it is crucial to comprehend the emerging epidemiological trends, mechanisms, and risk factors associated with these infections. To address this issue, a research study was conducted at PIC Peshawar, one of the largest cardiology hospitals in Peshawar. A total of 17 samples were collected using dry cotton tip swabs from various hospital equipment. These samples were then cultured on Sabouraud Dextrose agar media (SDA) for a period of 7 days. Sample identification was achieved using Bromophenol blue as a staining agent, while antifungal susceptibility testing was performed using Fluconazole. Direct microscopy was employed to isolate and identify 8 strains. These strains included Rhizopus sp., Penicillium spp., Aspergillus spp., Cladosporium sphaerospermum, Geotrichum candidum, Alternaria alternata, Scedosporium prolificans, and Scytalidium sp. Among these isolated colonies, Rhizopus sp., Aspergillus nidulans, and Aspergillus niger exhibited susceptibility to fluconazole, whereas the remaining colonies, Penicillium spp., Cladosporium sphaerospermum, Geotrichum candidum, Alternaria alternata, Scedosporium prolificans, and Scytalidium spp., displayed resistance to fluconazole. In our study, the highest fungal growth was observed in the pediatric ward (kids ward), indicating a need for targeted interventions in that area. The most frequently isolated fungal species included Cladosporium sphaerospermum and Aspergillus spp.